Agar Petri Dish Methodology, Technique and Where We're off to Next(P4/4 of a Two-Blog Series)

in STEMGeeks3 months ago (edited)

Welcome


Petri Dish Pouring - Methodology Technique, and Concluding Thoughts


Hello! And if you've reached this point, it is hopefully because of a desire to learn the final stages of petri dish preparation!


Continuing from yesterdays,
here,
https://hypnochain.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series

And last weeks,
Here:
https://hypnochain.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work
Based off :

And all the following, below;


While most petri dishes come prepared for bacterial work, it is always a good standard to follow disinfection procedures when working with new dishes. These have been outlined previously and are to be followed up to this point.


Now, continuing onward with the knowledge we have acauired on petri dishes,

20210122_090331.jpg

one should ensure decontamination of all surface surrounding is done. While air flow and circulation should already be consistent, one should disinfect the pouring table and anything surrounding thoroughly.

The individual should sanitize their self through -possibly- shower and usage of lab coat or fresh, cling-free, non-shedding clothing.

Safety glasses, hairnet, and latex or other gloves should be worn for the pouring of the dishes. In the case that a Sealed Air Box – a closed top containment for contaminant free and still air transfer – is not available for use, high sanitary measures should be followed to reduce contamination risk of dishes during pouring, as this is increased tenfold without protection from air contamination.

See below a properly setup agar pouring stattion,

IMG_20201119_120124 (1).jpg


Reviewed earlier in a shorter manner previously when examining how to prepare Agar, the same technique should be followed always when pouring to ensure consistency and sanitation.


Prepare a pouring station clear of any clutter that is not needed and clean the area with a disinfectant cloth.

Once the pouring station has been effectively set up, sterilized, and sanitized, the cooker can be released or pot, opened, releasing the pressure from inside.

If completed correctly with an appropriately sized measuring cup, pouring from the spout will be simple if no spillover has occurred.


The following instruction should be followed:



  • Lift the cup out safely, with an oven mitt if necessary, and place in the SAB, or begin immediately if none.

  • Lift the top of each dish, pouring in a slow circling motion and ensuring the bottom of the dish fills, while having no physical contact with any dishes.

  • Close each dish immediately after with the other hand and continue to pour the required number of dishes.

See below, a set agar dish from a pouring station for use with Agar medium;

IMG_20201119_120124.jpg

  • Seal each dish with parafilm or masking tape and store in the fridge or a cool place for use with the acquired spores.

Concluding, this ultimately is the final portion of my tri-section grouped blogging series on psilocybin and mycelium.

This section concludes day 2 of 2, part 4/4 of my blogging series. Initially, this may be a good introductory reading to those interested in mental health relatability: https://ecency.com/hive-125125/@trezzahn/a-cross-examination-of-the


If there is a genuine interest for those following these postings, this is something like you might get on your first cake :)

20210212_084035.jpg


You can begin section two through hypnochain, here:

https://stemgeeks.net/hive-163105/@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog

Or,

https://hypnochain.com/hive-163105/@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog


You can see yesterday's hypnochain's posting on syringes, freeze-storing, and other storage methods,
here:
https://hypnochain.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series
Or
https://stemgeeks.net/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series


You can see this past past weeks posting on Additionalities, Timeframes, and Where We're Off to Soon, here!
https://stemgeeks.net/hive-163521/@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part


Stemgeeks

https://stemgeeks.net/@trezzahn


You can see the second weeks posting on Agar utilization and proper methodology, with methodology including slicing and wedging, here :
https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series


And you can learn how to grow Agar, and how to prepare your dishes in the first weeks posting, here:


https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series,
Hypnochain @


https://hypnochain.com/@trezzahn



You can see yesterday's posting on storage methods, syringes, and other methods of maintaining quality,
here:
https://hypnochain.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series


You can see last weeks posting on Agar additionalities and utilization of liquid culture,
here:

https://hypnochain.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work


Agar medium and technique used second week,
here:

https://hypnochain.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series


as well as petri dish prep and an introduction to mediums used on such first week,
here:

https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series


And the initial introductory blog, here, going over the microbiology behind mycelium growth and bacteria:

https://hypnochain.com/hive-163105/@trezzahn/3f3eyz-psilocybe-mycelial-and-their-colonization-on-agar-agar
Hypnochain
https://stemgeeks.net/hive-163521/@trezzahn/psilocybe-mycelial-and-their-colonization-on-agar-agar
StemGeeks



my personal blog:

https://hive.blog/@trezzahn

and research blog:

https://ecency.com/@trezzahn/blog



PeakD:

https://peakd.com/@trezzahn

hive.buzz + hive.d.blog:

https://d.buzz/#/@trezzahn/t/buzz?ref=nav


Thanks again all for your interest!


Best regards,

#mushroom #psilocybin #psilocybe #mycelium #mycelial #petri #spore #psychedelic #psychoactive #hypnotic #hive #ecency #hypnochain #proofofbrain #stem #stemgeeks

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this is an awesome concept. the truth is that this tribe is good for learning but i have miss some lecture which i still need to go through the write up in other to learn previous topic.

The name Agar Petri Dish Methodology is new that I never heard of before but the technique is nice and good for learning.

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