Original Research: Evaluating the Healing Potency of Different Honey on Wound and Skin Infections Collected from Three Selected Hospitals in Nigeria

in StemSocial7 months ago (edited)

Interestingly, there are some natural products a lot of individual really don't know much about, this is with respect to the potency of these natural products. Back in the olden days, one would argue that our forefathers lived more healthier than us. Most of the things they used were mostly natural without any form of artificially produced chemical substance.

Fast-forward to this new age, a lot of diseases have been reportedly ravaging human existence up to the point that even microbials are now forming resistance to therapeutic drugs. The issue of drug abuse and drug resistance is one of the major problem facing the health care system. We are gradually beginning to drift from the use of natural products to more of synthetic, probably simply because the synthesics are usually more attractive.

There are some disease or problem that actually needs just a touch of nature to restore to normalcy. Honey is one natural product that has been widely reported to have huge benefits to humans. They possess great therapeutic potency, anti-inflammatory powers, Antioxidant and
above all, the ability to assist in rejuvenating damaged cells.

2000px-Manuka_honey_in_a_bowl.jpg
Manuka Honey

This research was done mainly to investigate some of the potency of honey and case in point l, its ability to heal skin and wound infection. This time around, our focus were hospitalized patients within few selected hospital in Nigeria. Let's begin the abstract and as usual, I will quote and elaborate on some areas when need be. Let's begin our discussion with the abstract of our work, shall we!

Abstract

The remarkable activity of honey varieties in inflammatory complications is increasingly favorable due to its ability to induce antibodies to produce cytokines (protein molecules that are involved in mostly involved growth and also play roles in cellular immunity) in eliminating bacterial infection and other related skin irritations. We investigated this assertion earlier documented in different studies about the potency of honey varieties in four selected hospitals within Nigeria. We also observed in this current work that the potency of honey obtained from different sources varied due to its composition of acids and proteins. Statistical technique, such as ANOVA was used to compare the mean difference of potency of honey in four selected hospitals within Nigeria. The test showed that the mean difference of the potencies of honey in three selected hospitals are not significant (P>0.05). The result of our invitro studies showed that honey had powerful antibacterial activities against different species of bacteria. We thus, concluded and suggested that among the countless varieties of honeys produced worldwide, some might have superior antibacterial activities that were yet to be discovered.

I would basically be limiting the discussion to the confirmed chemical composition of honey and why it very effective in prevention against microbials. We will look at its nature components in the context of how they are able effectively eliminate microbes which are the causative agents of infection. These microbes besides causing infection, prevent and slow wound healing and if they are not eliminated, they can cause increase in mortality rate.

Before we delve into discussing how honey plays crucial role in skin infection and wound healing, it is pertinent we understand the process of wound healing. By definition, wound healing is the process by which damaged tissues in a living organism are repaired through the replacement of the damaged tissues with a new one, it is as simple as that. It doesn't happen by magic, there are physiological processes that go on for it to occur. Anytime you are injured or recovering from infection that probably has wounds, has it ever occured to you how this processes occur. There are more to what you actually don't know. Reading this will put you ahead of others in knowledge. Let's get on with it shall we.

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Process of wound healing

The process of wound healing also referred to as tissue repair is basically made up of four stages namely:

•Hemostasis (blood clotting)
•Inflammation
•Proliferation (growth of new tissue)
•Maturation (remodeling).

The above must come into play in order for wounds to heal. Should there be any form of disruption to the process, it can results to delay in wound healing and if this happens, complications set in. The first stage which is Hemostasis (blood clotting mechanism) is made up of another substages that quickly act to arrest bleeding especially in cases that has to do uncontrolled bleeding. If the clotting mechanism is not initiated, wounds can never heal, so this is the reason why it is the very first thing that must happen before any other physiological process begin. In my previous article, I discussed about thrombosis (formation of blood clot inside blood vessels). Today we will now discuss how it happens externally. In Haemostasis, four things must happen and they are:

•Constriction of the blood vessel. •Formation of a temporary “platelet plug"
•Activation of the coagulation cascade
•Formation of “fibrin plug” or the final clot.

During the first stage of blood clotting system - Haemostasis, in a bid to arrest bleeding so that the patient doesn't pass our or loose excessive blood, the blood vessels that supplies blood to that region is restricted through a process called vasoconstriction (reduction in blood vessels internal diameter which leads to reduced blood flow). Once this is effectively done, next thing that happens is the formation of what is known as platelet plug. Recall from our previous article that platelets are mostly involved in coagulation processes, this time, they act to arrest bleeding.

In any case where an individual is lacking or has very low platelets concentration (thrombocytopenia), the chances of the wound healing process being delayed becomes very high. The alternative solution for these set of individuals would be to infuse them with platelets concentrates (This is just like transfusing blood to a patient but this time, you are giving the patient only platelets to enable the clotting process). When this is done, it is referred to as apheresis because you are giving the patient the major thing their body is lacking rather than just transfusing them with whole blood donates by others. This process in order words is strictly referred to as - platelet apheresis.

Now that the primary platelet plug has been formed, what next? The next thing that happens is the activation of the coagulation cascade system by different tissue factors. These stage is made of several clotting proteins known as clotting factors (Factors I to XII) that are produced by the liver and they all have different functions at every stage of the coagulation system. When any of these factors is lacking in an individual, it results to coagulation problem.

Coagulation_in_vivo.png
The coagulation cascade system

For example, an individual that lacks factor VIII is associated with a blood coagulation disorder known as Haemophilia A while those that lack factor IX is associated with Haemophilia B (also known as Christmas disease). The consequences of lacking clotting factors like factor 8 and 9 is that, they can bleed to death. So in essence, these individuals try as much as possible to avoid any scratch or any form of injury that would result to bleeding. In a nutshell, the type of blood clotting factor lacking in an individual will determine the type of blood coagulation disorder the person has and also the type of treatment to be followed.

Coagulation cascade system is made of both extrinsic and intrinsic pathways that are both activated differently but both still in any case leads to the formation of a more stable fibrin clot which is the last phase of haemostasis. So in essence, platelets help to produced a primary barrier to bleeding while the coagulation cascade does the major job of producing a fibrin clot that helps in stabilizing the barrier and making them more stronger and permanent per say.

If this doesn't happen, bleeding ensues. The clot seals the injured area, control and prevent further bleeding while the tissue regeneration process (wound healing) takes place. There is a very interesting thing that happens along the line, once the injury starts to heal, the plug or fibrin clot formed gradually begins to slowly remodels, and it dissolves with the restoration of normal tissue at the site of the damage and before you know it, your skin all looks alike and over time, you might not even be able to pin point the exact spot you once had injury.

Wound_healing_phases.png
Wound healing phases

So after Haemostasis, what next? This brings us back to stages of wound healing. Since the blood flow has been arrested, the next stage of wound healing will be for the body to initiate the Inflammation process. Inflammation is actually a good response to tissue injury sometimes, reason being that, it allows the movement and release of inflammatory chemicals (chemicals that will activate the attraction of phagocytic cells to the site of injury e.g interleukin 6) and white blood cells (neutrophils - phagocytic in function, i.e eat up other abnormal cells through the process of phagocytosis) and proteins (epidermal growth factors needed for regeneration of new tissue) to the site of injury. What role do all these substances release play?

Have you ever asked yourself, people with internal injury and bleeding, how are the dead blood cells cleared? The released chemical substance and cells all work in unison in helping to clear out all dead cells as well as bacteria and any other possible foreign substance or pathogen from the site.

Anytime inflammation begins, the soldiers cells (white blood cells) of the body are triggered and through the process of diakineses, they migrate to the site of injury to do their job. The said white blood cells also release their own chemical that cause continual flow of blood to the injury site for protection. This is the reason why you observe the redness and warmth in area of injury. Note also that, the reason ice is used on players that have sustained injury in the field is to reduce inflammation and enhance quicker recovery since the injury was not an open one. The ice block will reduce the swelling and also limit the migration of white blood cells to the area of injury.

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Pain reduction with the use of ice

If inflammation does not occur especially in an open injury, the chances of infection is high. So in essence, inflammation is protective in function. These is why whenever you are hurt or injured, a close examination of that area, you would experience these 5 cardinal features of pain - Calor (Heat), Dolor (Pain), Functio Leasa (Loss of Function), Rubor (Redness) and Tumor - (Swelling). I believe you now understand.

Note at this point that, it is not compulsory for you to feel all the above mentioned cardinal features of inflammation, it can actually go on saliently without your notice. After inflammation,the next stage of wound healing is the Proliferation stage (growth of new tissue).

Proliferation is the increase and growth of new cells. This is the actual stage that initiates the tissue repair and replacement of the damaged with new one through the action of endothelial cells which results to angiogenesis (development of new blood vessels to supply blood to the damaged region to Keep alive). There is also deposition of collagen (precursor for skin development), epithelialization (formation of new epithelial cells that gradually migrates to the top to protect the newly formed layer and finally there is wound Contraction.

During the stage of contraction, by mere looking at site of injury, you would visibly see it and that this stage, the possibility of scratching the area is high because of the mild discomfort it produces. This action is executed by a special type of muscle cells called myofibroblasts. They grip the edges of the wound during the process of contraction. After the whole processes, any cell that is no longer needed is marked for cell death and they consequently undergo apoptosis - natural cell death.

The last stage is the stage of remodelling (aka maturation). At this stage, the collagens are well realigned along tension lines and those cells that are also not needed ate eliminated though apoptosis. After all this stage, your skin or sure of injury is restored back to normalcy. With time the fully develops and match the normal skin colour of the individual. So those are the basic steps on how wound healing occurs and anything that can facilitate the process can be advantageous especially in cases of emergency and need for quicker recovery. This brings to back to our research topic.

Our research focused on studying the Healing Potency of Different Honey on Wound and Skin Infections Collected from Three Selected Hospitals in Nigeria. We aimed at finding out if there are difference in the potency of different varieties of honey by experimenting their potency on wound infection.

General overview of the Healing potency of honey and our research technique

Let's start with a general overview of honey's components and the roles they play. These substances are the power and engine room behind honey potencies.

Honey has its property and also varies in its chemical constituent on different types of varieties. It has remarkable effect and activity and has a lot of potent compounds used in the elimination of skin inflammation and wound complications.

Glucose oxidase which is one of the key component of honey is an enzyme that degrades glucose.

Glucose oxidase catalyses the oxidation of glucose to hydrogen peroxide. It is present in honey because the honeybees synthesize the enzyme and deposit it into the honey, where it acts as a natural preservative.

Honey provides glucose to leucocytes (white blood cells) which is essential for the respiratory burst (the rapid release of the reactive oxygen species, superoxide anion and hydrogen peroxide, from different cell types) that produce hydrogen peroxide which is suggested to give rise to its antibacterial activity and also possible elimination of microbes.

Many substances suspended in honey such as phenolic acids leads to its high acidic content and also increased pH. Amino acids (precursor for the synthesis of proteins generally) suspended in different honey varieties helps in regenerating tissues. If you would recall that amino acids are the building blocks or precursors for the synthesis of proteins in humans, so in essence it would be safe to say that, they play critical role in protein synthesis.

Since wound infection must be able to heal after eliminating of the microbials causing the infection (by actions of the phagocytic cells) and preventing it from healing, the presence of substance that contains the precursor for protein synthesis is just the perfect substance. Most people experience sharp pain after application of honey on affected lesions (infected or wounded areas), this is due to the acidic content of honey coming in contact with the nerve ending of the skin surface or lesions.

Research shows that different honey varieties have shown enormous potency in elimination of microbes such as bacteria and fungi with a distinction of proliferating cell tissue, regenerating tissue and also modelling of skin exposed surfaces. Cytokines are produced during wound healing process and also modelling such as clotting of blood as we have discussed above. Honey as an ant-inflammatory agent helps in slowing destruction to the cell and induces rapid and spontaneous healing process.

Proliferation of B-lymphocytes and T-lymphocytes in cell culture is stimulated by honey at concentrations low as 0.1%. In patients suffering from malignant wounds (of cancer origin), improvement with respect to wound size and cleanliness was seen after treatment with honey-coated bandages. Similarly, honey dressing quickened rates of healing in pressure wounds Honey has also been used to lessen foul odors emanating from wounds which cause a social barrier for patients and may lead to isolation. Therefore,gamma irradiation was introduced to destroy occasionally seen spores in honey, while having no adverse impacts on honey’s beneficial properties. In addition, honey dressing has economic advantages to the patient. Rapid healing reduces hospital stay and dressing material and surgical costs.

Results and findings

A total of 3 samples were used in order to assess their potencies respectively. Statistical techniques such as ANOVA is used to compare the Mean difference of the potencies of honey on wound and skin infections in three selected hospitals in
Nigeria.

Table-1: Shows the phenolic acidic levels (percentages) of different honey obtained from three selected Hospitals in Nigeria and Applied on wound surfaces; as shown below ;

Screenshot_20220109-165514_1.png

The test above is carried out as 5% level of significance F-ratio= 1.175, P- value or Ftabulated=6.93. The test above shows that the Ftabulated is greater than the F-ratio (Ftabualted > F-ratio)

We conclude that the mean difference between the healing potencies of different honey on wound and infection among three selected hospitals is not significant.

Table-2: An overview chart of different chemical constituent found in honey among three selected hospitals
Screenshot_20220109-170801_1.png

Table-3: Shows the average composition of honey (in g)
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Dicussions and Conclusion

In modern times, honey potency in medical field has been enormous in dealing with many wound and skin complication caused by microbes. Due to its acidic nature, many microorganisms are not seen wandering in its mixture but aids in elimination of certain microbes. Healing properties are in many areas even in traditional medicine for gastric ulcer and also in relieving pain. Manuka honey in recent times constitutes mostly of high acidic content, Jarrah honey
is used in ant-fungal inflammation of skin and so on.

Similarly, skin and wound inflammation is caused sometimes by itching and also chemicals that are introduced on the skin surface. Many of skin dreaded diseases in some parts of the world may respond negatively to chemicals used on them for repair and possible healing process. Honey constitute of about 200 substances, including amino acids, vitamins, minerals and enzymes, but it primarily contains
sugar and water. Sugar accounts for 95–99% of honey dry matter. The principal carbohydrate constituents of honey are fructose (32.56 to 38.2%) and glucose (28.54 to 31.3%) which speed the rate of healing due to its suspended acidic and chemical components. Medically, honey has been proven to eliminate microbes such as bacteria and fungi from the skin and also organ that have exposed lesion damages.

In conclusion, honey potency has shown increased distinction among other selected healing agents in the field of medicine. Production of hydrogen peroxide from glucose oxidase induces an enormous antibacterial properties found in honey which remove or sieve off bacteria. In field of medicine, honey has different varieties which show great potency on wound and skin inflammation.

There are countless varieties of honeys being produced worldwide, and some may have superior antibacterial activities. It can be concluded from in vitro studies that honey has powerful antibacterial activity against different species of bacteria. Many bacteria including aerobes (Those bacteria that require oxygen for growth) and anaerobes (They do not require oxygen for growth) are very sensitive to honey due to its acidic components. The substances which have about a pH of 6 and thus destroys most microbes.

Recent studies have assessed the useful and beneficial effect of honey on wound and skin inflammation with many methods that have been put in place to ensure proper use of honey varieties on wound and skin inflammation. Many possible finding and discoveries are still yet to unfold more of the healing potency of different honey and still require more of useful innovation and research inventions.

I Believe strongly that as more research and advancement continue in the area of medicine, more of the potencies of honey will be unraveled and harnessed in probably finding a permanent solution to antimicrobial resistance to synthetic drugs which Is now a major area of concern and problem with the currently used wide array of antimicrobials (drugs used in the treatment and eradication of microorganisms that cause infection in both humans and animals.

We can conclusively say that honey indeed has antimicrobial potency also aids in wound healing. In addition, there is not much of any clinical and significant difference in the different honey used for this research study with regards to their healing potency and constituents.

For details on the research methodology and full article download, these can be accessed here for free.

All images are free for use as the journal is an open access, just do well to reference us.

Thanks for reading

Until I come your way again.

References
Cardinal signs that you have an inflammation in Vacaville
Physiology, Haemostasis
The Five Cardinal Signs of Inflammation: Calor, Dolor, Rubor, Tumor … and Penuria (Apologies to Aulus Cornelius Celsus, De medicina, c. A.D. 25)
Glucose Oxidase in Honey
Hemophilia B
Blood Coagulation and Blood–Materials Interactions
The extrinsic pathway of blood coagulation
Traditional and Modern Uses of Natural Honey in Human Diseases: A Review

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Thanks a lot for another great post from you. I remember a recent post released on STEMsocial discussing the benefits of honey on burning injuries. And the scene in this post was also happening in Nigeria. Unfortunately, I cannot find it anymore… Maybe does @gentleshaid remember who wrote it or have the link?

Anyways, I am happy to read your post tonight, that goes deep into the topic with quantitative estimates (even if I had to wait for the end of the blog to get them), as well as with a detailed description of the healing process as a bonus which I did not expect (I didn’t know that much on the inflammation process by the way).

I will start my comment with a side note. I in fact especially enjoyed one of the very first sentences you wrote:

Most of the things they used were mostly natural without any form of artificially produced chemical substance.

Many people claim to try to live without relying on any chemical substance, which I find somewhat hilarious. They obviously refer here to artificially-produced substances. I appreciated the fact you insisted on this one word.

Now I have a real question/comment.

I didn’t know about the ANOVA method, which I looked on the Internet. In fact, it is probably quite close to methods I have used for my own research (i.e. a principal component analysis (PCA) aiming to investigate the variance of a feature space). The difference is probably that the PCA tries to combine feature, which is not the case here (as the dimensionality of the feature space is quite reduced).

By the way, this blog was much more accessible to people outside your field than the previous one I have read from you (on leukemia). I guess such a feedback could be useful to you (I am desperately looking for similar ones for my own texts).

PS: ahah, here is an open access article :D

😆
No matter how we want to say it, chemicals are inevitable. The said plants and herbs people consume even contains more and higher dose of phytochemicals ranging from Alkaloids to others. If not for dosage, the dangerous effects can be devastating.

ANOVA is one statistical tool that most of my associates love using Especially in cases of disparity and variance in values. It's much more friendly in this regards.

Most of my research works are actually Published under open Access, except textbook versions - the Publishers would hardly let it out for free...lol.

So sorry about your kid @gentleshaid , children at early age can be really troubled with recurrent ailments, but proper diagnosis will sure settle it. Hopefully he will sure bounce back hale and hearty.

Thank you sir

I guess the usage of this or that method is probably field-dependent. :)

I remember the honey post but can't really pick the author right now as my head is not really clear (currently in the hospital with my son on another admission).

I'm actually more familiar with ANoVA than PCA. It is my go to method for testing for significance. I only learned PCA briefly and never really build on it.

No problem for the honey post. It is clear you have more urgent and more important matters to deal with at the moment. Good luck!

How did I miss this? I am a fan of honey for wounds, so that is not surprising. The fascinating part of this article for me was the description of wound healing, especially where the wound contracts. I always noticed this with small scratches and wondered about it.

This action is executed by a special type of muscle cells called myofibroblasts. They grip the edges of the wound during the process of contraction. After the whole processes, any cell that is no longer needed is marked for cell death and they consequently undergo apoptosis - natural cell death.

There is something so willful about this. Our bodies perform all these processes all the time and we just go on year after year unaware of how amazing the whole thing is.

Thank for a great article.

Lol...there are more to even what we don't know that the body does for us humans.

As we research deeply, we tend to find out more.

I always appreciate your well articulated feedback.

Thanks for reading.

I am kind of curious. Did you use human subjects or just swabs from wounds? Your write-up is not explicit on this particular aspect. Your title says 'healing potency of honey on wound and skin infections. If you used swabs, it means what you investigated is the antibacterial potential of honey against wound isolates.

On another look, it may also be that you just analyzed the contents of the 3 honeys and then logically inferred their wound healing potential? I am really not clear on the exact methodology used in this research. perhaps a little clarification will work.

Please tag @gentleshaid when you respond to this comment.

Alright @gentleshaid

Did you use human subjects or just swabs from wounds?

Regarding this, human subjects were used not swab.

What we did was to apply the Honey indirectly on the wound lesions or inflammations (depending on the type of complications the patient has) by first applying it on the dressing. Applying the honey directly on the wound will result to spillage, hence the reason we applied it on the dressing before placing it on the patient.

We then left it on the dressing for 4 days as described by previous methodology used by other researchers to observe differences in the rate and speed of the healing Process. Observation was done by comparing them with human subjects serving as control.

Sorry if I'm sounding quite naive but I'm really yet to get it.

If I may get you right, human subjects with wounds were used as your treatment group. If that's the case, I don't see the relevance of using humans without wounds as control. The right thing, I stand corrected, would have been to compare the healing potency with conventional treatment excluding the honeys.

The patients with wounds should have been divided into 2 groups, exposed to the same treatment while the treatment group is given honey in addition. Then, the healing can now be compared after a chosen period.

No no no, @gentleshaid I think my explanation at the latter ending was not clear enough.

Human subjects with wounds were used as control obviously... probably the Way I constructed the English here got you confused.

Observation was done by comparing them with human subjects serving as control.

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The more I learn, the more I'm convinced that most diseases can be easily cured with proper diet and exercise. Some of the rest can be cured with simple home or traditional remedies such as the one you describe. Very few require professional medical intervention.

Example: For years I used to take an ointment and an eye drop every time I got uveitis; and the uveitis happened ~2 times a year, mostly when the seasons changed for the colder. Had to go to the eye doctor, get the prescription, and all that jazz. Now what I do is I cover my head with a hoodie for as much of the day as I can and I wash my eye with cotton dipped in black tea 3 times a day. Never needed the eye doctor since I started doing this. Is it possible that medicine is unaware of this? Hard to believe that some scientist hasn't described it in some paper that others willfully ignore or don't care to confirm with further research.

Haha, indeed actually some home remedy work better than synthetic drugs.

I am not a fan of most synthetic drugs, I love nature. The issue with using natural products without dosage is overdose.

Regardless, there are a lot of things science is yet to unravel in the area of medicine. I will dig deep to find out the relationship between the uveitis and the black tea, that will be a nice topic to research on. Harnessing the therapeutic potential will sure be a breakthrough.

Awesome!

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