Mango Kush Soil Microscope Analysis with Audio

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(Edited)

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Using a three inch apple corer, I took 3 core samples that were halfway in-between the edge of the fabric pot and the stalk of the plant. I placed the samples in a Ziplock bag and brought it back to my room/lab in order to prepare a sample.

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I used a dilution of 10 and two drops on the slide for my sample. First, I scanned for Nematodes at 100x total objective.

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Nematode Scan

I found 5 Bacterial Nematodes!

Next, using the same sample, I did a main assessment where I looked for filamentous organisms and protozoas at a 400x total magnification.

Starting at the top right area of the 18x18 coverslip and going from left to right in a straight line, I chose 5 random areas in that lane to read the different Fields of View in each area.

The following videos are Five readings in consecutive order.

Reading 1

Reading 2

Reading 3

Reading 4

Reading 5

Now that I did my main assessment and scanned for nematodes, it is time to make a new slide just for my bacterial counts. I will dilute this sample to a dilution factor of 1000 and use 2 drops on the slide.

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In five different areas, much like the 5 side of a dice, I will record 5 different areas on the cover slip to count for bacteria. Since there was lots of bacteria count I counted a 1/4 of the screen/cover slip area at 400x total magnification.

Bacterial Count Readings

Results

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While I did not really see any fungi to write to home about, I did see a crapload of flagellates and bacterial feeding nematodes. Which is a really good thing. I did see one ciliate on my nematode scan which is still concerning. The bountiful amount of flagellates is apparently signs the soil might be over watered as flagellates favor aquatic environments over soil but still are great nutrient cyclers. The ciliate is concerning but watering has been tricky as I can only water once a week, too wet, as well as there being a compaction layer under each fabric container. Do remember that this grow is 20 feet away from a creek so perhaps the extra flagellates are also feature of growing in this area.

So a really good reading in my opinion. Seems my soil here has a lot of nutrient cycling going on!

Are you curious about the microscope and using one for soil analysis? Check out my Soil Food Web mentor Wes in a recent Soil Food Web video about using the microscope!



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9 comments
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Everything can be approached either scientifically or by trial and error. The scientific method sometimes requires so much time and resources that the latter is more useful...

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Doing God’s work! Hey what nematode will be good for gnats. And when will I see results after adding them?

!1UP

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They literally have attempted my murder and are trying to kill me with V2K and RNM. Five years this has been happening to me, it started here, around people that are still here. Homeland security has done nothing at all, they are not here to protect us. Dont we pay them to stop shit like this? The NSA, CIA, FBI, Police and our Government has done nothing. Just like they did with the Havana Syndrome, nothing. Patriot Act my ass. The American government is completely incompetent. The NSA should be taken over by the military and contained Immediately for investigation. I bet we can get to the sources of V2K and RNM then. https://ecency.com/fyrstikken/@fairandbalanced/i-am-the-only-motherfucker-on-the-internet-pointing-to-a-direct-source-for-voice-to-skull-electronic-terrorism ..... https://ecency.com/gangstalking/@acousticpulses/electronic-terrorism-and-gaslighting--if-you-downvote-this-post-you-are-part-of-the-problem if you run into one of them you may want to immediately shoot them in the face. 187, annihilate, asphyxiate, assassinate, behead, bleed, bludgeon, boil, bomb, bone, burn, bury, butcher, cap, casket, choke, chop, club, crucify, crush, curb, decapitate, decimate, deflesh, demolish, destroy, devein, disembowel, dismember, drown, electrocute, eliminate, end, euthanize, eviscerate, execute, explode, exterminate, extinguish, finish, fry, grind, guillotine, gut, hack, hang, hit, ice, implode, incinerate, kill, liquidate, lynch, massacre, maul, microwave, mutilate, neutralize, obliterate, off, pop, poison, punnish, quarter, ruin, shank, shock, shoot, shred, skin, slay, slaughter, smoke, smother, snipe, snuff, squish, stab, strangle, stone, suffocate, suicide, SWAT, swing, terminate, torture, terrorize, whack, waste, wreck. You better fucking kill me.

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Quite a well executed lab procedure, I would love to have my own lab at home, my research center is inoperative due to vandalism they stole the electrical system, and for now I am not using the lab. I enjoyed your publication.

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(Edited)

Like woah. When you said school I thought you meant curriculum school. Class is in session—noted!

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