Cold-Stored Mycelial Transfer - Petri, Agar, and Transfer Methodology

in Fungi Lovers4 months ago (edited)

Welcome to my summary of this weeks work on petri dish utilization via transfer and isolation of cold-stored psilocybin multi-strained dishes!

This may sound confusing, but, simply put, there were three days of work and essentially three major steps to this process:

  • Clean, Sanitize, and Sterilize Dishes
  • Prepare and Pour Agar Agar, and
  • Cut cold-stored Petri storage colonies to these newly prepared Agar Petri Dishes.

So, without further adieu, let's begin!

Wednesday, January 12th 2022


This day was spent preparing my dishes for cleaning.

20220110_181207.jpg

After receiving,

20220112_004333.jpg

I prepared my work station,

20220112_193025.jpg

With utilization of Clorox after a soap and water clean and rinse for both 2 minutes, 99% Isopropyl Alcohol is used to soak the dish for 15 seconds.

20220112_200140.jpg

The dishes cleaned well, and were ready after a nights dry to be used tomorrow!

IMG_20220113_121813.jpg

Yay! Past Section 1.1.1 and 2 of my tek, this is seen in Section 1.2: - https://ecency.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Thursday, January 13th 2022


Onward! Today I prepared Agar! I used my reagent ingredient recipe found here - https://ecency.com/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series
And following proper procedure, stove-top cooked my Agar Agar recipe to create Agar -

20220113_140556.jpg

Such for use pouring in my own now dried Petri dishes -

20220113_165536.jpg

And letting these now poured Agar dishes cool overnight until the following day!

Friday, January 14th 2022


Lastly, these now poured Agar Dishes are cooled and ready for use!

20220113_165518.jpg

These are prepared for use transferring previously cold-stored Petri's to these new dishes - something done every 6 months to ensure viability -

IMG_20210302_121526.jpg

And as these prepared dishes are cut,

IMG_20220114_124411.jpg

according to my tek Section 1.3.2 found here - https://ecency.com/hive-163521/@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part
I transfer, beautifully, two Golden Mammoth - Ajax, two Golden Capped - Penis Envy and One P. Cyanescens to these five prepared Agar Petri's;

20220114_163429.jpg

Concluding my three day journey this week working with psilocybin mycelium on Agar Petri Dishes, I ask the question.

What has everybody else been up to this week?

Stay safe, stay somber!

Update: See next weeks work here https://ecency.com/hive-166168/@trezzahn/cold-stored-mycelial-transfer-part-two-petri-agar-and-transfer-methodology--examination-of-last-weeks-work

Trezzahn



Check out some of my other stuff!

https://peakd.com/@trezzahn

https://ecency.com/@trezzahn/blog

https://ecency.com/hive-125125/@trezzahn/from-spore-to-mushroom-colonizing-consolidating-and-birthing-fresh-psilocybin-ready-for-flush-and-harvest-final-edit-full-guide


Support a healthy lifestyle!




#hive #hypnochain #hypno #stemgeeks #stem #proofofbrain #blog #photography #blogging #steem #steemit #ecency #psychedelic #hypnotic #mushrooms #psilocybin #mycelial #mycelium #petridish #agar #agaragar #beautiful #photogenic #enjoyment #life #adventures #beauty #happy #trezzahn

Sort:  

hey there! excellent post -- thank you for sharing your know-how. hope it may be useful to all mates who are in fungi cultivating business. supported by FL community account and nominated for #OCD curation
ps. we have a MushroomGrowers community at Hive, but sadly is not active.

Heya @hive166168 ! Thanks for the reply!

I intend to help others with their own journeys into the realm of therapeutic psilocybin usage!

Hopefully some more can find this post of good use! :)

ofc, therapeutic. 🙆‍♂️ sure thing!

Yay! 🤗
Your content has been boosted with Ecency Points, by @hive-166168.
Use Ecency daily to boost your growth on platform!

Support Ecency
Vote for new Proposal
Delegate HP and earn more

You've been curated by @trucklife-family on behalf of The Herbal Hive.We support any content related to herbalism, from stories about your relationship with medicinal plants, to wildcrafting, foraging, ethnobotany, plant spirituality & herbs as nourishment, as well as the use of natural products to create medicines such as salves and tinctures. Use the tag #theherbalhive or post from our community!
Community II Delegate II Curation TrailII Discord

divider.png

Thank you! Much appreciated!

added your post to the bookmarks. tho I did not grow mushrooms, I guess it can be useful someday -- how one can know in advance?

I agree! It is a useful knowledge to learn about, not only for psilocybin, but other fungi too!

Thanks for the reply @qwerrie

PS. have you ever tried the tag #proofofbrian? try it, you may like the result 😁

Thankfully I did for this post :)