Cold-Stored Mycelial Transfer - Petri, Agar, and Transfer Methodology

in Fungi Loverslast year (edited)

Welcome to my summary of this weeks work on petri dish utilization via transfer and isolation of cold-stored psilocybin multi-strained dishes!

This may sound confusing, but, simply put, there were three days of work and essentially three major steps to this process:

  • Clean, Sanitize, and Sterilize Dishes
  • Prepare and Pour Agar Agar, and
  • Cut cold-stored Petri storage colonies to these newly prepared Agar Petri Dishes.

So, without further adieu, let's begin!

Wednesday, January 12th 2022

This day was spent preparing my dishes for cleaning.


After receiving,


I prepared my work station,


With utilization of Clorox after a soap and water clean and rinse for both 2 minutes, 99% Isopropyl Alcohol is used to soak the dish for 15 seconds.


The dishes cleaned well, and were ready after a nights dry to be used tomorrow!


Yay! Past Section 1.1.1 and 2 of my tek, this is seen in Section 1.2: -

Thursday, January 13th 2022

Onward! Today I prepared Agar! I used my reagent ingredient recipe found here -
And following proper procedure, stove-top cooked my Agar Agar recipe to create Agar -


Such for use pouring in my own now dried Petri dishes -


And letting these now poured Agar dishes cool overnight until the following day!

Friday, January 14th 2022

Lastly, these now poured Agar Dishes are cooled and ready for use!


These are prepared for use transferring previously cold-stored Petri's to these new dishes - something done every 6 months to ensure viability -


And as these prepared dishes are cut,


according to my tek Section 1.3.2 found here -
I transfer, beautifully, two Golden Mammoth - Ajax, two Golden Capped - Penis Envy and One P. Cyanescens to these five prepared Agar Petri's;


Concluding my three day journey this week working with psilocybin mycelium on Agar Petri Dishes, I ask the question.

What has everybody else been up to this week?

Stay safe, stay somber!

Update: See next weeks work here


Check out some of my other stuff!

Support a healthy lifestyle!

#hive #hypnochain #hypno #stemgeeks #stem #proofofbrain #blog #photography #blogging #steem #steemit #ecency #psychedelic #hypnotic #mushrooms #psilocybin #mycelial #mycelium #petridish #agar #agaragar #beautiful #photogenic #enjoyment #life #adventures #beauty #happy #trezzahn


hey there! excellent post -- thank you for sharing your know-how. hope it may be useful to all mates who are in fungi cultivating business. supported by FL community account and nominated for #OCD curation
ps. we have a MushroomGrowers community at Hive, but sadly is not active.

Heya @hive166168 ! Thanks for the reply!

I intend to help others with their own journeys into the realm of therapeutic psilocybin usage!

Hopefully some more can find this post of good use! :)

ofc, therapeutic. 🙆‍♂️ sure thing!

Yay! 🤗
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You've been curated by @trucklife-family on behalf of The Herbal Hive.We support any content related to herbalism, from stories about your relationship with medicinal plants, to wildcrafting, foraging, ethnobotany, plant spirituality & herbs as nourishment, as well as the use of natural products to create medicines such as salves and tinctures. Use the tag #theherbalhive or post from our community!
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Thank you! Much appreciated!

added your post to the bookmarks. tho I did not grow mushrooms, I guess it can be useful someday -- how one can know in advance?

I agree! It is a useful knowledge to learn about, not only for psilocybin, but other fungi too!

Thanks for the reply @qwerrie

PS. have you ever tried the tag #proofofbrian? try it, you may like the result 😁

Thankfully I did for this post :)