The Simple Lab: How Do I Make My Mushroom Spawn At Home?

in Fungi Lovers5 months ago

Have you thought, what if mushroom you have seen you could have your own in your home? Have you been interested in planting mushrooms but don't know to start, or don't know yet how to make some? Well, eunoia is here for you, giving you insights into how you could make a spawn, your spawn, even if you are not in the expensive laboratory or just in your home.

To understand what spawn is…

A spawn is a group of mycelium, mycelia are a group of spores, and a spore is a seed of a mushroom. Not scientifically as seed, but spore is the reproductive features, where one single spore could replicate its own. When they form a group, they become mycelia, and this is a white web-like fungus phase that wants to eat what they could encounter. Spawn is an active mycelium that grows in the seed medium.

In terms of business, mushroom spawn covers 50% of your expenditure, so when you master making your own, you will also lessen the input by 50%. But still challenge because it's a very brilliant job, a task that not everyone can do, but in my case, I do my own. As of now, I will share with you how.

In the Philippines, you could buy some mushrooms with 100 grams of spawn for 100 pesos or $2. A kilo is worth 1000 pesos or $20, quite expensive. So better to make your own.


I am doing the "Agar isolation technique" the idea is that brings the nutrient mycelia need in jelly "agar-agar" I make my own by adding two agar-agar bars (transparent color), one litter of distilled water, 20 grams of plant-based dextrose powder.


  1. One liter of distilled water must boil. After the boiling, place the agar-agar bar and dextrose powder and stir well until they dissolve.
  2. Place the solution into the bottle. A small amount could so. One litter of liquid could have 20 bottles or more. The more battle, the more success you have.
  3. Cover the bottle with cotton balls and bring it to the pressure cooker(autoclave) for exactly 30 minutes. The count must start when the pressure weasels.
  4. Place the hot bottles immediately in the chamber and avoid touching them with your bare hands. Then wait until it cools down..


This is the stage where you must clean yourself before doing the course. Everything must be clean from your head to toe, fingernails must be cut and removed the dirt, hair must be tied up, the facemask is a must, and plastic gloves must be in the proper position. You are like a doctor who will open up wounds; even single debris might cause severity and contaminations in your spawnings.

The first and primary stage is to look for healthy specimens, not more than one day, and not be exposed to or touched by humans and insects. Carefully remove them from the substrate you have already been sanitized with rubbing alcohol and place them to cover them to avoid more contaminations. After looking for a candidate mushroom, please do not touch it frequently unless you sanitize your hands before and after doing so.

To trap the mushroom spore, split it in half, and from the center of the tail and gills, use a blade or scalp that has been sanitized to get a trim and tiny piece of mushroom flesh. Immediately place it on the agar mixture.

Carefully place the flesh and bring it to the center of the bottle. You can also use a petri dish if you are working in a laboratory, but I am making my own with my DIY, not forgetting the science behind it. So even a bottle could be used just that we must disinfect and cleans several times.

This will be what the mushroom looks like within 24 hours of trapping its spore. As the nutrient from the agar-base jelly is absorbed, they will release a spore, and as soon as they form in mycelia in the following hours.

This is what we called Mycelia after three weeks of trapping. They will build a colony of spores as we could preserve them in the manner of years or centuries to come. I have a lot of this as I stored and sleeping soon I shall wake them up when the mood comes. But I have been a bit inspired as I wanted to blog. Maybe I will also make a blog and what was progress daily for you to figure out.


After waiting a month to trap the spores and build the mycelia, it's time to make a spawn.

Mushroom spawn is made using grains such as wheat, barley, and sorghum but is quite expensive, so I looked for alternatives and built my technology and style in preparing it. I am using rice grains, corn, and seeds. Rice and corn are abundant here, and I might get it free.


  1. Cleaning the grains several times, ten times is what I am doing. Drain the water and air dry until reaching a 40% water content when you use corn grits and 60% in rice grains. That is what I discovered in my trials and self-research.
  2. After air drying, applies a concentrated lime, not an agricultural line. Concentrated is the higher form of calcium that will maintain the pH level of the grain.
  3. Then place it in the bag or bottle and sterile it using a pressure cooker or autoclave for about 30 minutes.
  4. Then place it in the incubation chamber.

The incubation chamber helps to minimize the affecting foreign fungi while doing the task, as some of the contamination is in the air. An incubation chamber is highly needed and increases the chances for a successful grain of mushrooms.

Here is what it looks like when you successfully make a grain spawn. It was created by corn crits and is about three weeks old.

And here are the rejected spawns, which are contaminated, using contaminated spawns resulting mush in some problems. It might not be the fungi or mushrooms you planted that will come, so I don't use contaminated spawn. It's waste, but it's part of the process.

This is ready-to-use spawn, a healthy and no other color that signifies success in culturing your mushroom.

As I said, mushroom spawn is also available in the market. However, due to the hard-to-make one, price still varies in different names such as F0 (Filial Zero or the PDA generation), F1 (Filial One- First generation), until F10. But doing my own and your own will decrease your expenditure but takes time to master.

Next week we will discuss the materials you need to have in mushroom cultivation. Lets's talk about the edible mushroom. Thanks for reading…



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I was always curious about how people grow fungi, and your post was a perfect read for me... I have learned something new! Thanks for that!

I have picked this post on behalf of the @OurPick project which will be highlighted in the next post!

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Thank you very much, as I inspired me to write more things about mushroom and fungi from the limit that I know.

Wow, napaka-delicate Pala mag alaga Ng mushrooms...Inaabangan ko na siya kase gusto kontalaga mag alaga sa loob Ng Bahay kaso baka kapag lumamig na Muna panahon Dito sa unit..Alam ko kase mamatay sila pag mainit ata..

Opo, when its too hot there could be chances soon I will discuss it and how to prevent it din.

I feel like I've learned something new hehehe, because of the community where I live, many mushrooms grow 👀

Wow this is so warm in my heart knowing that someone learn something, especially that was my goal in the first place. Thank you.

Nuon pa man isa ka ng alamat! Thanks for sharing this one @eunoia101 . It so amazing how you put everything in order. But it takes a lot of time pala nuh!

Yes po, need a time and attention in producing one.

Galing..kumusta na yung Oyster Mushroom business mo?

Nakatulog Ms, jane haha soon bubuhayin ko ulit nang ma blog ko more whahaha,

Export mo dto, benta ko sa market 😂. Fav yan mga chikwa haha

Interesting! I've been fascinated by mushroom farms and I watched a lot of YouTube videos before. It takes a lot of care to control the variables for mushroom growth, but I always find it fascinating. In terms of market, I think the Philippines is not really keen into mushrooms. Do you sell your produce or it's just for consumption?

That's how I also starts from watching YouTube then reading a book, and yeah sadly Philippines is not yet when it comes to mushroom. But today's as more and more products had been made surely the venture will bloom. I do mine for experience but some had been market in locality hehe. Neighborhood muna.

Yeah! I figured that the Philippines is not yet ready for mushrooms, but if you have a steady customer from the neighborhood, that would be amazing.

Galing mo naman @eunoia101 and I can see you having a great company soon having mushroom as the product hehehe.

Naalala ko asawa ko, may nakita daw sya parang web ng spider sa lab kung saan sya nagtatrabaho. Akala nya ano, yun pala maging mushroom daw yun.

soon having mushroom as the product

Soon ate hehe this will happened, I will build my name in such success very soon hehe.

daw sya parang web ng spider

oww maybe it was a mushroom spawn.

Thanks ate for visiting.

Go @eunoia101, make it happen. I will be happy to know,to see you having it in the future. I will be your supporter.

I love the mushroom, but I got distracted with your pretty face. Anyway, back to the mushroom, there's a lot of process pala. It is complicated for me to understand hehe.

haha pretty face is nothing, its on genetic so soon my Child face is better added with your chromosome Jiji whahaha.

There's a lot to learn from your blogs talaga sir eu lalo na pinag-iisipan ko na mag cultivate din ng mushrooms kahit konti lang sana ng matry dito..

uyy why not, try mo bro read and learn and if something you might not understand talk to me agad.

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Yung friend nang asawa ko may ganitong business eh.. I mean bibili tulad nang ginawa mo tas papatubuin ang galing pero mdjo may kamahalan capital nya non..

Anyway congrats sayo laki na nang mga tips hohoho. Sana all.

Oo grabe laki capital, so I make myself DIY as much possible. Truely mahal ng mga materyales. Yang ganyan seed 1k per kilo na huhu.

Kaya nga eh, nag invite kasi yung friend nang asawa ko na mag ganyan nga kaso ang mahal wala pa kami budget.. pero malaki din daw naman kitaan lalo pag walang ibang nag supply nang mushrooms sa lugar.

Pag iisipan ko to kung gusto ko magbusiness ng mushroom hehe pero mukhang for consumption na lang siguro. Adu met gamien ditoyen isu nga kasla saturated ti market.

Congrats! Galing ng mga posts mo. Informative and easy to follow. Keep it up!

Kaya nga po, maganda sa inyu at alam na ng tao some people here still hesitant sa mushroom haha. Uric acid, eme and daming issues.

Thankyou for sharing this Eunoia. ♥️ I've learned a lot so much from you!💓 Keep it up Eunoia, so proud of you.

Thank po, more to learn hehe and I will try to explain it more for sure.

I can see your success in this field. Keep going.

Yiee nandito Kana pala sir Bjorn hehe. Salamat po.

Ang cute nung second pic parang ano lang hehe charot. Ang galing mo naman mag alaga ng mushroom boi, feeling ko tlga ito magiging malaking business mo in the next coming years.

Naman, haha but I could picture myself na magturo ba, so I also could learn more hehe. I also want other varieties eh.

Pwede yan yonong pwede ka maging prof sa agri!! 🥰🙌

Galing galing naman

meanwhile I'm just curious if you keep all those spawns at home or if you go to a lab or something because I'm worried about fungal infections ;;w;; tho mycelia is becoming a meat substitute. You could make mushroom bacon with all of those mycelia

Haha they build me mini lab actually yieee... But now becomes storage hahaha.. soon bubuhayin ko

Ohh cutieee
Who built that tho? Parents?