"Birth" of a Daphnia🧠👶/ "Parto" de una Daphnia📚🔬

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If I don't do this experiment you would probably think that the microscopic world is very simple "because it is not similar to us" but in reality it is more complex than you imagine! In this post we are going to observe "a water flea under controlled conditions" and we will see how the babies develop inside it and at the same time we will see how complex this group of animals is!📚🔬🧠

Si no hago este esperimento probablemente pensarias que el mundo microscopico es muy simple "porque no es parecido con nosotros" pero en realidad es mas complejo de aquello que imaginas! en este post vamos a observar "una pulga de agua en condiciones controladas" y veremos como se desarrollan los bebes dentro de ella y al mismo tiempo veremos cuan complejo son este grupo de animales!👨‍💻🤯

Specimen characteristics👨‍🏫🧠/ Caracteristicas del ejemplar🙇‍♂️📚

Well, to understand a little about how my little experiment worked, I extracted from stagnant fresh water an adult specimen of: (Daphnia) whose species I identified as: (Daphnia logispina), if I calculate it does not fail me, it means about: (2 millimeters), therefore it is an animal that is not microscopic because we could see it swimming in the water due to its "large size", the advantage of studying this specimen of Daphnia is because they are translucent and light passes through them easily, which makes it easier for us to see and count the embryos , make an estimate and this female that I named "lulu" had 19 eggs" as I captured it in a pond they end up being translucent but the color can vary depending on the water conditions!🤔👨‍🏫📚
Bien para entender un poco sobre como funciono mi pequeño experimento extraje del agua dulce estancada un ejemplar adulto de: (Daphnia) cuya especie identifique como: (Daphnia logispina), si me calculo no me falla media unos: (2milimetros), por tanto es un animal que no es microscopico porque podriamos verlo nadar en el agua por su "gran tamaño", la ventaja de estudiar este ejemplar de Daphnia es porque son traslucidos y la luz lo atraviesa facilmente lo que nos facilita la posibilidad de ver y contar los embriones, realice un estimado y esta hembra a la que bautice como "lulu" tenia 19 huevos" como lo capture en un estanque acaban por ser traslucidas pero el color puede variar dependiendo de las condiciones del agua!🤯👨‍💻🔬

The incubation period of water fleas is fascinating and they must make up to five "molts in their exoskeleton" to reach sexual maturity! Under normal conditions with a temperature between 20-28 degrees these animals make several changes in the same day, and the process happens between 15-20 minutes before laying a group of eggs! when I captured "lulu" she already had her fertilized eggs but they were very small and I only appreciated her "compound eyes" here in the video you can see the first stages of incubation of the water flea, as they are filter feeders the water where it was had enough "detritus" and algae so that it could be fed, it was with me about 3 days in the test tube.🤯👨‍🏫🧠
El periodo de incubacion de las pulgas de agua son fascinantes y deben realizar hasta cinco "mudas en su exoesqueleto" para alcanzar su madurez sexual! en condiciones normales con una temperatura entre los 20-28grados estos animales hacen varias mudas en un mismo dia, y el proceso sucede entre los 15-20 minutos antes de poner un grupo de huevos! cuando capture a "lulu" ella ya tenia sus huevos fecundados pero eran muy pequeños y solo apreciaba sus "ojos compuestos" aqui en el video puedes ver las primeras etapas de incubacion de la pulga de agua, como son filtradores el agua donde estaba tenia bastante "detritus" y algas para que se lograra alimentar, estuvo conmigo unos 3 dias en el tubo de ensayo.🤯🙇‍♂️🙇‍♂️

Life expectancy👨‍🏫🧠/ Esperanza de vida🙇‍♂️📚

These animals are (parthenogenetic) a large part of the year and this indicates that a large population of (Daphnia logispina) are female during the year, since they can also reproduce asexually, the ovary has direct communication with "the oviduct" , which in turn communicates with the incubation chamber! When the eggs arrive from the oviduct to the incubation chamber, they begin to develop until they complete their embryonic development! From what I was able to observe this species: (Daphnia logispina) has a "direct post-embryonic" development, that is, when the offspring leave the mother they are fully developed and independent!🧠👨‍💻🔬
Estos animales son (partenogenéticos) una gran parte del año y esto nos indica que una gran poblacion de (Daphnia logispina) son hembras durante el año, ya que pueden reproducirse tambien de forma asexual, el ovario tiene una comunicacion directa con "el oviducto", que a su vez se comunica con la camara de incubacion! cuando los huevos llegan del oviducto a la camara de incubacion comienzan a desarrollarse hasta que culminan su desarrollo embrionario! por lo que pude observar esta especie: (Daphnia logispina) tiene un desarrollo "post-embrionario directo" osea cuando las crias salen de la madre estan desarrolladas completamente y son independientes!🤯👶

It is difficult to estimate the embryonic state of these animals as they vary between species and environmental conditions! "lulu" my female water flea had an embryonic development of 24 hours maybe a little less and her eggs were a little developed so I had to make an estimate! and coincides with the cited work where the "embryonic development of C. reticulata and D magna lasted 16 hours and 24 hours"🔬🙇‍♂️
Es dificil estimar el estado embrionario de estos animales ya que varian entre especies y condiciones ambientales! "lulu" mi pulga de agua hembra tuvo un desarrollo embrionario de 24 horas quizas un poco menos ya sus huevos estaban un poco desarrollados asi que tuve que realizar una estimacion! y coincide con el trabajo citado donde el "desarrollo embrionario de C. reticulata y D magna tuvieron una duracion de 16 horas y 24 horas"👨‍🏫📚🧠👨‍💻

Last day and birth👨‍🏫👶/ Último día y nacimiento🙇‍♂️📚

It is very difficult to draw a good conclusion about the work since I don't know if under conditions of stress for the animal the eggs hatch faster or slower, it is something that few scientists analyze in their work! but the fact is that almost all of them were born alive, I would say 98% of all were fit to swim and looked good! the mother died after a few hours concidence? or stress? More than a scientific approach, seeing the microscopic birth of an animal that is "almost invisible" is a good opportunity for me to show you how complex the animal kingdom is!🧠👨‍💻
Es muy dificil sacar una buena conclusion sobre el trabajo ya que no se si en condiciones de estress para el animal los huevos nacen mas rapido o mas lento es algo que poco cientificos analizan en sus trabajos! pero el hecho es que casi todos nacieron vivos, diria que el 98% todos estaban aptos para el nado y tenian buen aspecto! la madre murio pasado unas horas concidencia? o estress? mas que un enfoque cientifico ver el nacimiento microscopico de un animal que es "casi invisible" es una buena oportunidad que tengo para mostrarle a ustedes cuan complejo es el reino animal!🙇‍♂️🔬📚

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Sources /Referencias bibliograficas:

  1. https://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000400007

  2. Dialnet-HistoriaDeVidaDeDaphniaMagnaYCeriodaphniaReticulat-4732822.pdf

  3. Atlas de los microorganismos streble y krauter (1985)

  4. https://www.croa.com.ar/la-daphmia-pulga-agua/

Due to the scientific nature of this post, the descriptive material was extracted from the pages cited for direct citations: ""

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I like so much all these images you share with us (and the story behind them). Cheers!

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