*Updated* Mycelial Storage - Syringes, Cold-Storing and other Methods (P4/4 of a Two-Blog Series)

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(Edited)

Welcome

Syringes, Freeze-Storing, Culturing, and Shelf-Life - A Final Examination

Spore/Inoculated Dishes and Spore Syringes - Cold Storage:

To be completed upon development of,

  • a liquid colony that is both fully cultured and contaminant free in a stored mason jar to be sealed and/or cold-stored,

  • a directly flame-sterilized injection syringe inoculation of a new spore dish [via utilization of a liquid culture and its’ associated spores], or a dish that be colonized fully; to be sealed and cold-stored, or,

– This colonized dish (possibly also from injection via liquid culture and withdraw with 1/10/100cc injection syringe) to be cold-stored, ensuring proper technique for parafilm wrapping is utilized for dishes;

Depending on the medium of storage; syringes and dishes are to be stored in a plastic bag, and jars with tape sealing the injection hole, stored in a cold dark place. For short-term storage.

  • For longer, a plastic container should be sanitized and sterilized, as per guidelined laid out earlier, and petri dishes placed individually into plastic ziploc bags, and wrapped into this container. See below, dishes prepared to be cold-stored.

IMG_20210302_121526.jpg

  • The container is to be stored in a crisper, cooler temperature (vegetable crisper, for example) and the jars, post-wrapping, can be stored the same, sometimes able to last years with the mycelium growth halted.

  • It is often common practice to maintain a liquid culture in a commonly shook jar for its' 6 months of efficacy only then transfer to anew not soon after - so it is advisable to follow this to save your growth!

Furthermore, although methodology to acquire these forms of storage may be long, storage time of up to two years allows a large variety and ease of use whenever desired. Worthwhile!

IMG_20210214_113259.jpg

While both techniques have benefits, determinate time versus actual speed; overall effectiveness versus exterior factors affecting; ease of methodology versus difficulty of practice, etc., it should be noted that contamination is always a key environmental factor, and that sterilization is very important to exhibit during early stages of growth whenever your developed colony is exposed to open air.

Once colonization gets to a certain point, it becomes evident that a once small spore, now a living and breathing element, will continue to grow and adapt to conditions, and be difficult to stop, like one would see in the environment where the certain mycelium are naturally found.

See below, a properly wedged and colonized dish, and or some to be cold stored;

IMG_20210223_103326.jpg

20210115_124716.jpg

And above, the result of our next few months work - We'll get here!

Upon sterile inoculation, there will begin to be evident signs of this growth within 7-14 days, - contamination, even earlier – and full colonization of this substrate within 2-3 weeks following.

From this point, liquid spore can be withdrawn from the honey-culture liquid and freeze-stored in a sealed ziploc bag in a crisper, similar to above.

Life! Exciting!

Concluding, this ultimately is the halfway portion of my tri-section grouped blogging series on psilocybin and mycelium.

This section concludes day 1 of 2, part 4/4 of my blogging series. Initially, this may be a good introductory reading to those interested in mental health relatability: https://ecency.comhive-125125/@trezzahn/a-cross-examination-of-the

Updated Links (for Ecency {+app} and PeakD)

Ecency

Starting Out, and Psychoactive Research

/@trezzahn/psychoactive-research

or

https://ecency.com/steemgeeks/@trezzahn/psychoactive-research

Medical Benefits - Writing (Articulate)

/@trezzahn/a-cross-examination-of-the

or

https://ecency.com/hive-125125/@trezzahn/a-cross-examination-of-the

Outline Postings

HypnoChain Posting

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or

https://ecency.com/hive-163105/@trezzahn/general-hypnochain-outline-month-following

StemGeeks Posting

/@trezzahn/petri-dish-technique-and-methodology-month-following

or

https://ecency.com/hive-163521/@trezzahn/petri-dish-technique-and-methodology-month-following

Introduction to Psilocybin

HypnoChain Posting

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or

https://ecency.com/foo/@trezzahn/3f3eyz-psilocybe-mycelial-and-their-colonization-on-agar-agar

StemGeeks Posting

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or

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Section 1

Part 1.1

/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series

or

https://ecency.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series

Part 1.2

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or

https://ecency.com/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series

Part 2.1

/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

or

https://ecency.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Part 2.2

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Part 3.1

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or

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Part 3.2

/@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part

or

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Part 4.1

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or

https://ecency.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series

Part 4.2

/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series

or

https://ecency.com/hive-163521/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series

Section 2

Part 1.1

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Part 1.2

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or

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Part 2.1

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or

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Part 2.2

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Part 4

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or

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PeakD

Starting Out

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Psychoactive Research

https://peakd.com/steemgeeks/@trezzahn/psychoactive-research

Medical Benefits - Writing (Articulate)

https://peakd.com/hive-125125/@trezzahn/a-cross-examination-of-the

Outline Postings

HypnoChain Posting

https://peakd.com/hive-163105/@trezzahn/general-hypnochain-outline-month-following

StemGeeks Posting

https://peakd.com/hive-163521/@trezzahn/petri-dish-technique-and-methodology-month-following

Introduction to Psilocybin

HypnoChain Posting

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StemGeeks Posting

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Section 1

Part 1.1

https://peakd.com/hive-163105/@trezzahn/an-introduction-to-spore-growth-and-reproduction-in-a-controlled-environment-p1-4-of-in-a-two-blog-series

Part 1.2

https://peakd.com/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series

Part 2.1

https://peakd.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

Part 2.2

https://peakd.com/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series

Part 3.1

https://peakd.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work

Part 3.2

https://peakd.com/hive-163521/@trezzahn/mycelium-growth-preparation-agar-additionals-general-timeframes-and-further-work-the-fun-part

Part 4.1

https://peakd.com/hive-163105/@trezzahn/mycelial-storage-syringes-cold-storing-and-other-methods-p4-4-of-a-two-blog-series

Part 4.2

https://peakd.com/hive-163521/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series

Section 2

Part 1.1

https://peakd.com/hive-163105/@trezzahn/mycelial-caking-and-growth-an-introduction-to-colonization-mediums-a-continuation-of-section-1-section-s-2-p1-1-4-in-a-two-blog

Part 1.2

https://peakd.com/hive-163521/@trezzahn/brown-rice-flour-and-rye-grain-substrate-a-scientific-examination-of-its-utilization-as-a-medium-for-mycelial-growth-a

Part 2.1

https://peakd.com/hive-163105/@trezzahn/mycelial-consolidation-and-colonization-of-a-substrate-medium-a-continuation-to-colonization-mediums-cont-section-1-section-s-2

Part 2.2

https://peakd.com/hive-163521/@trezzahn/shotgun-fruiting-chamber-s-construction-use-and-utilization-a-continuation-of-section-2-p2-2-4-in-a-two-blog-series

Part 3.1

https://peakd.com/hive-163105/@trezzahn/birthing-of-consolidated-mycelium-cakes-pailocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series

Part 3.2

https://peakd.com/hive-163521/@trezzahn/fruiting-and-growth-of-birthed-mycelium-cake-psilocybin-growth-a-continuation-of-section-2-p3-4-in-a-two-blog-series

Part 4

https://peakd.com/hive-163521/@trezzahn/harvest-and-flush-of-birthed-mycelium-cake-substrate-psilocybin-growth-a-conclusion-of-section-2-p4-4-in-a-two-blog-series

my personal blog:

https://hive.blog/@trezzahn

and research blog:

https://ecency.com/@trezzahn/blog

PeakD:

https://peakd.com/@trezzahn

hive.buzz + hive.d.blog:

https://d.buzz/#/@trezzahn/t/buzz?ref=nav

Thanks again all for your interest!

Best regards,

Old Links

You can see tomorrow's posting on agar petri dish methodology here;
https://stemgeeks.net/hive-163521/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series
or,
https://hypnochain.com/hive-163521/@trezzahn/agar-petri-dish-methodology-technique-and-where-we-re-off-to-next-p4-4-of-a-two-blog-series

Based off week one's :

And all the following, below;

Stemgeeks

https://stemgeeks.net/@trezzahn

You can see the second weeks posting on Agar utilization and proper methodology, with methodology including slicing and wedging,
here :
https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-agar-and-its-utilization-working-with-it-proper-technique-and-further-use-p2-4-in-a-two-blog-series

You can learn how to grow Agar, and how to prepare your dishes in the first weeks posting,
here:
https://stemgeeks.net/hive-163521/@trezzahn/an-introduction-to-petri-dish-preparation-for-use-with-growth-medium-day-two-p1-4-in-a-two-blog-series,

Hypnochain @

https://hypnochain.com/@trezzahn
You can see last weeks posting on Agar additionalities and utilization of liquid culture,
here:

https://hypnochain.com/hive-163105/@trezzahn/liquid-culture-what-it-is-methodology-and-colonization-and-further-work

Agar medium and technique used second week, here:

https://hypnochain.com/hive-163105/@trezzahn/mycelial-spore-utilization-on-agar-petri-dishes-p2-4-in-a-two-blog-series

#mushroom #psilocybin #psilocybe #mycelium #mycelial #petri #spore #psychedelic #psychoactive #hypnotic #hive #ecency #hypnochain #stem #stemgeeks #proofofbrain

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