How does alcohol react with the chloroplast?🧠 / alcohol+chloroplast🔬🧫

Hello science lovers, remember your chemistry classes when the teacher told us that ethanol and other alcohols that have a hydroxyl group are organic compounds that are soluble in water, do not have coloration, and have a characteristic odor! and today in the industry they are used in a specific concentration as antibacterial! Now, if they are organic compounds, it means that in some way they could affect the chemistry-biology of living beings, yesterday while I was treating my plants I thought it would be interesting to do an experiment to see how ethyl alcohol affects plant tissue, join me in this experience !🧠🔬

Hola amantes de la ciencia, recuerdan sus clases de quimica cuando la profesora nos comentaba que el etanol y otros alcoholes que tienen un grupo hidroxilo son compuestos organicos que son solubles en el agua, no tienen coloracion y tienen un olor caracteristico! y hoy en dia en la industria son utilizados en una concentracion especifica como antibacterianos! ahora bien si son compuesto organicos quiere decir que de alguna forma podrian afectar la quimica-biologia de los seres vivos, ayer mientras trataba mis plantas pense que seria interesante hacer un experimento para ver como el alcohol etilico afecta el tejido vegetal, acompaname en esta experiencia!🧫☘️

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Scientific article🧠/ Articulo cientifico🧫🔬

To carry out an experiment as viable as possible, I will use as a reference the scientific work of the author: (Elvimer, 1993) which uses various types of alcohols in plant tissue to measure all possible alterations. For my experiment I will use only ethyl alcohol with ethanol: ( 70%), for the biological samples I will carry out the experiment with three different species of leaves: (all aquatic) I think that using plants of different species will help to see how long it takes for the alcohol to penetrate the cuticle, I mean, I suppose that the thicker it is it will be more difficult for ethanol to react! But hey, that's the theory, the practice will give us another result! I will use a small glass container, tweezers, and a blade to make the cuts!🔬🧫🧠
Para realizar un experimento lo mas viable posible utilizare como referencia el trabajo cientifico del autor: (Elvimer,1993) el cual utiliza varios tipos de alcholes en tejido vegetal para medir todas las alteraciones posibles, para mi experimento utilizare unicamente alcohol etilico con etanol: (70%), para las muestras biologicas realizare el experimento con tres especies diferentes de hojas: (todas acuaticas) creo que usar plantas de diferentes especies ayudara a ver cuanto demora el alcohol en penetrar la cuticula, osea me supongo que mientras mas gruesa es sera mas dificil el etanol reaccionar! pero bueno esa es la teoria la practica nos arrojara otro resultado! utilizare un pequeno envase de vidrio, pinza, y hojilla para hacer los cortes!👨‍🎓🕵️‍♂️☘️🧪

http://cidbimena.desastres.hn/RMH/pdf/1993/pdf/Vol61-1-1993-4.pdf

Ethyl alcohol🧠/ Alcohol etilico🧫🔬

I want to highlight that aquatic plants do not have cuticles or stomata because they do not need any support tissue because they are submerged! in them it is easier to see the chloroplasts and that is why I used them in the experiment but some have thicker structures and you can see it under the microscope! for this I used a "measure" "density" by this I mean that it is the thickness of the leaf and we can see it with the penetration of light, the faster the light from the microscope passes and shows me the organelles then it will be less dense! If it is very dense, the light will not pass through easily and we will only see "dark" which would be plant tissue!☘️🕵️‍♂️
Quiero resaltar que las plantas acuaticas no presentan ni cuticula ni estomas debido que no necesitan ningun tejido de soporte porque estan sumergidas! en ellas es mas facil ver los cloroplastos y fue por eso que las utilice en el experimento pero algunas tienen estructuras mas gruesas y lo puedes observar en el microscopio! para ello utilice una "medida" "densidad" con esto quiero decir que es la espesura de la hoja y podemos verla con la penetracion de la luz, mientras mas rapido la luz del microscopio pasa y me muestra los organelos entonces sera menos densa! si es muy densa la luz no pasara con facilidad y solo veremos "oscuro" que seria tejido vegetal!🧫🧠👨‍🎓

Light (macro and micro)🔬🧠

To better see the effect of alcohol on the leaf, we will use the two types of micro lights: (macro and micro) one to illuminate the external plant structure, and the micro light that passes through the leaf. Believe me, we will see very interesting things with both lights. ! Starting the experiment I will make a small cut of the leaves submerged in water.🌍🧠

para ver mejor el efecto del alcohol en la hoja utilizaremos los dos tipos de luces del micro: (macro y micro) uno para iluminar la estructura vegetal externa, y la luz del micro que atraviesa la hoja creeme que veremos cosas muy interesante con ambas luces! comenzando el experimento realizare un corte pequeno de las hojas sumergidas en agua.🧠

There are three ways in which alcohol can affect the membranes: firstly by altering the fluidity of the membrane this in turn will affect the proteins and cause a "functional error" in the enzymes and channels! another effect on the plant would be that a "dehydration would also occur at the level of the membranes" and last and most interesting to see in the microscope: "a direct interaction with the proteins" that is, we are going to see live how alcohol enter the membrane and discover the chloroplasts and after many hours only the cell will remain without any organelles and the alcohol will turn green!🔬🧠👨‍🎓
Existen tres formas en las cuales el alcohol puede afectar las membranas: primeramente alterando la fluidez de la membrana esto a su vez va afectar las proteinas y producira un "error funcional" en las enzimas y los canales! otro efecto en la planta seria que ocurriria una "deshidratacion que tambien se daria a nivel de las membranas" y por ultimo y mas interesante de ver en el microscopio: " una interaccion directa con las proteinas" osea vamos a ver en vivo como el alcohol entra en la membrana y descubre los cloroplastos y con muchas horas solo quedara la celula sin ningun organelo y el alcohol se tornara verde!🕵️‍♂️☘️🧪

alcohol can react with other polar molecules, but they also have the characteristic of being hydrophobic and therefore can react in hydrophobic regions in lipids or proteins, producing an alteration in them! what alcohol does in plant tissue is to produce an alteration in bone fluidity, it becomes "more fluid", in plants it is important to maintain an adequate fluidity for the flow of proteins, and when alcohol makes the membrane more fluid proteins begin to present alterations! this because it also affects lipids!👨‍🎓🧠
el alcohol puede reaccionar con otras moleculas polares, pero tambien tienen la caracteristica de ser hidrofobicas y por tanto pueden reaccionar en regiones hidrofobicas en los lipidos o proteinas lo que produce una alteracion en las mismas! lo que el alcohol hace en el tejido vegetal es producir una alteracion en la fluidez osea se vuelve "mas fluida", en las plantas es importante que se mantenga una fluidez adecuada para el flujo de proteinas, y cuando el alcohol hace de la membrana mas fluida las proteinas comienzan a presentar alteraciones! esto porque tambien afecta a los lipidos! 🕵️‍♂️🔬

Alcohol effect🧠/ Efecto del alcohol🧫🔬

We can summarize the effect of alcohol on the leaf as a "fixer", that is, the chemical process is that the chloroplasts dissolve in the alcohol and it will turn it green! alcohol is an organic solvent, it makes the leaf permeable as explained above and the chloroplast is discovered, which in turn dissolves in the alcohol.🧠🕵️‍♂️
Podemos resumir el efecto del acohol en la hoja como un "fijador", osea el proceso quimico es que los cloroplastos se disuelven en el alcohol y lo tornara verde! el alcohol es un solvente organico hace que la hoja se vuelva permeable tal como lo explique anteriormente y se descubre el cloroplasto que a su vez se disuelve en el alcohol. 🧫☘️

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According to the article that I used as a reference, the alcohol concentration is important to see the effects of the fluidity of the leaf, as I used a 70% ethanol concentration, we clearly see how in a few minutes the leaf loses its properties and begins to look less green product than alcohol is dissolving chlorophyll! The question is what happens after many hours? What happens to chloroplasts and chlorophyll?🕵️‍♂️🧠
Segun el articulo que utilice como referencia la concentracion de alcohol es importante para ver los efectos de la fluidez de la hoja, como yo utilice un 70% de concentracion de etanol claramente vemos como en pocos minutos la hoja va pierdiendo sus propiedades y comienza a verse menos verde producto que el alcohol esta disolviendo la clorofila! la pregunta es que sucede luego de muchas horas? que sucede con los cloroplastos y la clorofila?🕵️‍♂️🧠🧪

After several hours the leaf is completely "clear" we do not see green pigmentation and this because when we take a photograph with more than 100x we do not see the chloroplasts? and what happened? Let's say the chemistry did its job, what then happens is that it dissolved in the alcohol and as you can see the alcohol now looks green, the chlorophyll is now in the alcohol molecules.🕵️‍♂️☘️🧠
Luego de varias horas la hoja queda totalmente "clara" no vemos pigmentacion verde y esto porque cuando hacemos una fotografia con mas de 100x no vemos los cloroplastos? y que sucedio? digamos que la quimica hizo su trabajo lo que a continuacion sucede es que se disolvio en el alcohol y como puedes ver el alcohol se observa ahora verde, la clorofila esta ahora en las moleculas del alcohol.🔬🧫

benefits of alcohol🧠/ Beneficios del alcohol🧫🔬

It is not all bad news, there are some scientific studies that show that alcohol could be the future to combat drought. In an experiment, it was shown that plants that are sprayed with alcohol have a better probability of surviving stress and help to sustain themselves better in times of long droughts incredible right?🧫🔬

no todas son malas noticias hay algunos estudios cientificos que demuestran que el alcohol podria ser el futuro para combatir la sequia en un experimento se demostro que las plantas que son rociadas con alcohol tienen mejor probabilidad de sobrevivir al estress y ayuda a sostenerse mejor en epocas de largas sequias increible no?🕵️‍♂️🧪

https://www.catalunyapress.es/articulo/ciencia-e-investigacion/2022-08-26/3866821-alcohol-podria-solucion-afrontar-sequia

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I have never tried that before. I should keep this in mind for enrichment activities.

!discovery 41

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The photos here are really good. You reminded me that I need to learn so much more in chemistry.

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