Haematococcus pluvialis culture🧠👨💻🥽🔬 (En-Es)
Hello friends addicted to science, growing algae is something that requires a lot of technical patience and knowledge of microbiology, and for the first time I enter the world of creating an artificial system to cultivate algae in optimal conditions for its growth, even though red algae are the species. "Haematococcus pluvialis" is actually a green algae with a high content of "astaxanthin" which in turn is rich in carotenes, therefore giving it that very beautiful bright red color, however we must know that the main objective of the algae when secreting the Astaxanthin is to protect your "photosystem" What do you think if you join me in this experience??👨💻🙇♂️🔬
Hola amigos adictos a la ciencia cultivar algas es algo que requiere mucha paciencia tecnica y conocimiento de microbiologia, y por primera vez me adentro al mundo de crear un sistema artificial para cultivar las algas en condiciones optimas para su crecimiento, apesar que algas rojas la especie "Haematococcus pluvialis" en realidad es un alga verde con un alto contenido de "astaxantina" que a su vez es rico en carotenos por tanto le da ese color rojo vivo muy bonito, sin embargo debemos saber que el principal objetivo del alga al segregar la astaxantina es para proteger su "fotosistema" que te parece si me acompañas en esta experiencia¿??📚👨🏫🕵️♀️
My home experiment🔬🧠/ Mi experimento casero📚🥽
I took the water sample from a small puddle in a rainwater deposit area where wastewater probably reaches. The water is fresh and was around 20 degrees in normal environmental conditions. When I brought it to my small laboratory, I subjected the algae to various conditions. environmental conditions such as temperatures ranging from 22 degrees to 26-27 degrees. Temperature is important in the growth of this algae, which resists 22-27 degrees. To increase the level of "pollution" and nutrients in the water, I added several drops of fertilizer. of algae that is rich for the growth of green algae. I measured the temperature daily with a homemade thermometer, and with strips to measure water parameters I managed to bring the water to the conditions I needed.🙇♂️👨💻🧠🌏
La muestra del agua la saque de un pequeño charco en un area de deposito de lluvias donde probablemente lleguen aguas residuales, el agua es dulce y rondaba los 20 grados en las condiciones ambientales normales, al traerla a mi pequeño laboratorio someti las algas a varias condiciones ambientales como temperaturas que oxilaban los 22 grados hasta 26-27grados, la temperatura es importante en el crecimiento de esta alga que resiste a los 22-27 grados, para incrementar el nivel de "contaminacion" y nutrientes en el agua añadia varias gotas de fertilizante de algas que es rico para el crecimiento de algas verdes. Diariamente media la temperatura con un termometro casero, y con tiras para medir parametros del agua consegui llevar el agua a las condiciones que necesitaba. 🥽👨🏫🥼⚗🧬
Colony growths 3 days🔬🧠/ Crecimientos de colonias en 3 dias📚🥽
This is a summary of what the experience was like and how the algae evolved within the artificial system. As you can see, it was a seven-day experiment where the color of the water varied due to the growth of the algae and the nutrients added to the water. The first days the water was at a temperature of 22.5 degrees and as the days passed the temperature increased until reaching days 5-7 where the water was at 26.4 degrees, so we see that the warmer the Temperatures grew faster and we can see the bubbles that are actually colonies of thousands or millions of algae, something I noticed was that the conjugated and unicellular green algae appeared more in initial conditions with not as many nutrients as the days went by they disappeared! I think it could be inferred because they did not receive direct sunlight naturally and the lamp was not enough for them to carry out their photosynthesis processes.⚗🌏🧠
Este es un resumen de como fue la experiencia y como evolucionaron las algas dentro del sistema artificial, como puedes ver fue un experimento de siete dias donde el color del agua iba variando por el crecimiento de las algas y por los nutrientes agregados en el agua, los primeros dias el agua estaba a una temperatura de 22,5 grados y a medida que pasaban los dias incrementaba la temperatura hasta llegar a los dias 5-7 donde el agua estaba a 26,4 grados, por lo que vemos que mientras mas calor las temperaturas crecian mas rapido y podemos ver las burbujas que en realidad son colonias de miles o millones de algas, algo que note fue que las algas verdes conjugadas y unicelulares aparecieron mas en condiciones iniciales con no tantos nutrientes con el pasar de dias fueron desapareciendo! creo que podria inferir por no recibir sol directo natura y la lampara no era lo suficiente como ellas realizar sus procesos de fotosintesis.👨💻🔬🕵️♀️
Dominant algae within 3 days🔬🧠/ Algas dominantes entre los 3 dias📚🥽
Here we see some species that were predominant during the first days when the temperature was around 22-24 degrees and little nutrient added to the water, as you can see, many of them are common in waters with a high level of solar incidence and stagnant contaminated water, however they left. disappearing, I suppose due to the lack of capacity to receive natural sun and the lamp was not enough to keep them alive! However, at this point the environment was very favorable for my Haematococcus pluvialis, which, despite also being a green algae, is very resistant, so much so that injecting large concentrations of alcohol incredibly did not destroy them.🕵️♀️🔬👨💻🥽
Aqui vemos algunas especies que eran predominantes entre los primeros dias cuando la temperatura rondaba los 22-24grados y poco nutriente añadido al agua, como puedes ver muchas de ellas comunes de aguas con alto nivel de incidencia solar y agua contaminada estancada, sin embargo se fueron desapareciendo que supongo por la nula capacidad de recibir sol natural y la lampara no fue suficiente para mantenerlas vivas! sin embargo en este punto el ambiente era muy favorable para mis Haematococcus pluvialis el cual apesar de ser tambien un alga verde es demasiado resistente tanto asi que inyectandole grandes concentraciones de alcohol increiblemente no se destruian.🌏⚗🧬
Occurrence of Haematococcus pluvialis🔬🧠/ Aparición de Haematococcus pluvialis📚🥽
Haematococcus pluvialis is a true survival green algae, when conditions are not favorable it enters a "cyst" state there it produces high concentrations of astaxanthin, this carotene helps protect the algae from ultraviolet rays and I wonder if I should use the UV lamp 24 hours a day. per day did the algae enter that phase? It could be... Other records determine that astaxanthin produces when there are no nutrients in the environment or there is little water, I don't think nutrients because it regularly uses oxygen and fertilizers, they are not common in warm or tropical climates, on the contrary, they are from temperate zones and They form in ponds!🥽📚🙇♂️
Haematococcus pluvialis es una verdadera alga verde de supervivencia, cuando las condiciones no son favorables entra en un estado de "quiste" alli produce altas concentraciones de astaxantina este caroteno ayuda a proteger la alga de rayos ultravioletas y me pregunto sera que utilizar la lampara UV 24h por dia hizo que la alga entrara en esa fase? podria ser... Otros registros determinan que produce la astaxantina cuando no hay nutrientes en el medio o hay poca agua, nutrientes no creo porque utilice regularmente oxigeno y fertilizantes, no son comunes de climas calidos o tropicales por el contrario son de zonas templadas y se forman en charcas! 🌏👨💻🔬
https://es.wikipedia.org/wiki/Haematococcus_pluvialis
The description of the alga is characteristic for being biflagellar, it is clolophyte because it contains both types of chlorophyll a and b, the white substance that surrounds it that looks like a "slime" is the starch it produces as a reserve substance, it can reproduce both sexually and asexually. It is so resistant that it has resistance to antibiotics such as "ampicillin". According to reports, this species has been recorded on all continents of the planet except Antarctica! She is a true survivor!🌏🙇♂️📚
La descripcion del alga es caracteristica por ser biflagelar, es clolofita porque contiene los dos tipos de clorofila a y b, la sustancia blanca que la rodea que parece una "baba" es el almidon que produce como sustancia de reserva, pueden reproducirse tanto como sexual como asexualmente, es tan resistente que tiene resistencia a antibioticos como: "ampicilina" segun los reportes se ha registrado esta especie en todos los continentes del planeta menos en la antartica! es una verdadera sobreviviente!🧠🕵️♀️🌏
https://es.wikipedia.org/wiki/Haematococcus_pluvialis
Super colonies🔬🧠/ Super colonias📚🥽
On the seventh day we see millions of algae that are in their "cyst" state. It seems that too much sunlight makes them turn red. It was a quite interesting experience. We can also see the whiteness of the bottom due to the starch used to consume it when the conditions of water are not favorable!🌏🕵️♀️📚
ya al septimo dia vemos millones de algas que estan en su estado de "quiste" al parece mucha incidencia de sol hacen que se tornen rojas, fue una experiencia bastante interesante tambien podemos ver lo blanco del fondo que el almidon utilizado para consumirlo cuando las condiciones del agua no son favorables!👨💻🔬
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